Abstract
Sephadex LH-20 has been substituted with hydroxycyclohexyl residues to give a stable gel for use in lipophilic gel chromatography. The gel can be used for straight-phase and reversed-phase systems. Separations of a model set of steroidal compounds are described. Hydroxysteroids epimeric at C-3, C-7, C-11, C-17 or C-20 have been separated as the free alcohols in a straight-phase system. Five ▶ 5-steroids have been separated from the corresponding dihydrosteroids of the 5α-series.
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