Preparation and controlled drug release ability of the poly[N-isopropylacryamide-co-allyl poly(ethylene glycol)]-b-poly(γ-benzyl-l-glutamate) polymeric micelles

Abstract

The polymeric micelles were prepared through a copolymerization of allyl polyethylene glycol (APEG) and N-isopropylacrylamide in the presence of 2-aminoethanethiol (AET), followed by a ring opening polymerization of γ-benzyl-l-glutamate N-carboxyanhydride (BLG-NCA). Doxorubicin (DOX) as a model drug was covalently conjugated into the core of micelles via hydrazone bonds. The drug loading capacity could reach up to 15% with drug encapsulation efficiency of 80%. The pH/thermo sensitivities were observed in the process of in vitro drug release. The DOX-loaded micelles exhibited accelerated drug release behaviors in an acidic condition, and enhanced therapeutic efficacy was observed. Furthermore, the cytotoxicity of micelles against Hela and 3T3 cells was evaluated before and after drug loading. The DOX-loaded micelles showed strong cytotoxic activity to the cancer cells. But the blank micelles showed non-cytotoxicity. Therefore, the thermo/pH dual-responsive polymeric micelles have a promising future applied as a controlled drug delivery system for anticancer drugs.