Abstract
The application of molecular imprinting technology in the separation and purification of active ingredients in natural products was widely reported, but remains a challenge. Enrichment and separation are especially limited. A surface imprinting technique was reported to synthesize molecularly imprinted microspheres (MIMs) in this article. With paeoniflorin (PF) as the template molecule, β-cyclodextrin (β-CD) and acrylamide (AA) as the functional monomers, and poly(glycidyl methacrylate, GMA) microspheres (PGMA) as the backing material. MIMs have been characterized by FTIR and FESEM. Adsorption experiments indicated the adsorption capacity of MIMs was superior to those comparative non-imprinted microspheres (NIMs) and the binding isotherm of MIMs was in good agreement with the two-site binding model. The baseline separation of PF and its structural analogue albiflorin (AF) were achieved on the new MIMs packed column. MIMs showed good affinity and efficiency for separation of PF and AF compared with those comparative NIMs. The approach of fabricating MIMs is simple, rapid, and inexpensive, and may shed new light on the application of MIMs as a liquid chromatography stationary phase to separate and analyze PF and AF from the Red peony root extracts.
Highlights
Paeoniflorin (PF, CAS: 23180-57-6) is a monoterpene glycoside, which mainly exists in radix paeoniae rubra and radix paeoniae alba [1]
PF and AF could be separated by baseline. These results indicate the possibility of applying molecularly imprinted microspheres (MIMs)-1 as the stationary phase to analyze or separate the Red peony root extracts
To separate PF and AF, a new approach for the fabrication of the PF MIMs was presented by molecular imprinting
Summary
Paeoniflorin (PF, CAS: 23180-57-6) is a monoterpene glycoside, which mainly exists in radix paeoniae rubra and radix paeoniae alba [1]. Molecular imprinting technique is a rapidly developing technique for preparing highly cross-linked polymeric materials that contain highly specific recognition sites and with predetermined selectivity to template molecules [11]. Because MIPs can selectively rebind the template or its analogs from a matrix, they have been extensively applied in solid-phase extraction (SPE) [13] and chromatography [14]. It is difficult to obtain MIMs with high affinity binding sites and selectivity with the preparation methods mentioned above, even those methods have significantly improved the adsorption properties of MIMs. β-Cyclodextrin (β-CD) was a kind of cyclic oligosaccharide with a hollow cylindrical structure it has a hydrophilic exterior and an internal hydrophobic cavity [19]. The MIMs exhibited excellent PF chromatographic performance, such as good separation of PF and its structural analogue albiflorin (AF) and larger adsorption capacity. This research might provide a new approach to the further separation and purification of natural products from natural product extracts
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