Preparation and characterization of polyclonal and monoclonal antibodies against fusarenon‐X

Abstract

Polyclonal and monoclonal antibodies against fusarenon‐X were prepared by using a fusarenon‐X oxime derivative coupled to human serum albumin as the antigen for the immunization of rabbits and BALB/c mice, respectively. The specificity and sensitivity of these antibodies were tested by using fusarenon‐X oxime coupled to horseradish peroxidase as an enzyme‐linked toxin in a competitive assay with a double antibody solid phase. The relative cross‐reactivities of the polyclonal antiserum with T‐2 toxin, diacetoxyscirpenol, fusarenon‐X and neosolaniol were 3.67, 2.75, 1.0 and 0.58, respectively. The monoclonal antibody, however, showed considerable cross‐reactivity only with neosolaniol (0.28). The detection limit for fusarenon‐X was 150 pg/ml (5 pg per assay) and 300 pg/ml (10 pg per assay), respectively, using the polyclonal and monoclonal antibodies.