Preparation and Characterization of Nano-Selenium Decorated by Chondroitin Sulfate Derived from Shark Cartilage and Investigation on Its Antioxidant Activity.

Jianping Chen,Baozhen Luo,Tugui Fan,Jiarui Li,Rui Li,Xiaofei Liu,Bingbing Song,Xuejing Jia,Xuehua Chen,Saiyi Zhong

doi:10.3390/md20030172

Jianping Chen, Baozhen Luo + Show 8 more

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https://doi.org/10.3390/md20030172

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Abstract

In the present study, a selenium-chondroitin sulfate (SeCS) was synthesized by the sodium selenite (Na2SeO3) and ascorbic acid (Vc) redox reaction using chondroitin sulfate derived from shark cartilage as a template, and characterized by SEM, SEM-EDS, FTIR and XRD. Meanwhile, its stability was investigated at different conditions of pH and temperatures. Besides, its antioxidant activity was further determined by the DPPH and ABTS assays. The results showed the SeCS with the smallest particle size of 131.3 ± 4.4 nm and selenium content of 33.18% was obtained under the optimal condition (CS concentration of 0.1 mg/mL, mass ratio of Na2SeO3 to Vc of 1:8, the reaction time of 3 h, and the reaction temperature of 25 °C). SEM image showed the SeCS was an individual and spherical nanostructure and its structure was evidenced by FTIR and XRD. Meanwhile, SeCS remained stable at an alkaline pH and possessed good storage stability at 4 °C for 28 days. The results on scavenging free radical levels showed that SeCS exhibited significantly higher antioxidant activity than SeNPs and CS, indicating that SeCS had a potential antioxidant effect.

Highlights

Reactive oxygen species (ROS) are the result of the normal cell metabolism of living organisms
In order to evaluate the effect of Chondroitin sulfate (CS) concentration on the formation of selenium-chondroitin sulfate (SeCS), the particle sizes of SeCS prepared with different concentrations of CS were measured by nanoparticle size analyzer
The average particle diameters of SeNPs were significantly decreased to 113.7 ± 2.89 nm in the presence of 0.1 mg/mL CS, indicating that CS between 0.05 mg/mL and 0.1 mg/mL could inhibit the aggregation of SeNPs with the increase of CS concentration

Summary

Introduction

Reactive oxygen species (ROS) are the result of the normal cell metabolism of living organisms. When the production of ROS exceeds the scavenging capacity of living organisms, oxidative stress occurs [1]. Excessive reactive oxygen species (ROS) affect normal metabolism, and is closely related to the occurrence and development of various diseases, including cardiovascular diseases [2], Alzheimer’s disease [3], nonalcoholic fatty liver disease [4], and so on. As an essential micronutrient for human health, selenium (Se) plays a vital role in protecting against oxidative stress [5]. The beneficial and harmful doses of Se is an extremely narrow margin, which limits its practical applications in food and medicine [6]. It is necessary to take effective measures to solve this problem

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