Preparation and characterization of microwave irradiated pH-sensitive polyacrylamide grafted flax seed mucilage graft copolymeric hydrogel (PFLSM-g-PAM-cl-MBA) and its evaluation as effective polymeric scaffold

Abstract

Microwave irradiated free radical synthesis of polyacrylamide-flax seed mucilage graft copolymeric hydrogel (PFLSM-g-PAM-cl-MBA) was evaluated for pH sensitivity at pH 2, 8 with maximum swellability at pH 7.4.The analytical characterizations of the optimized grade G6 was done using FTIR spectroscopy, 13C-NMR, TGA, XRD which indicated that grafting of acrylamide was successfully done on flax seed mucilage (PFLSM). The elemental composition also indicated successful grafting of acrylamide on flax seed mucilage. The electrokinetic potential of the G6 indicated it to be a good colloid stabilizer when compared to PFLSM. The surface morphology of G6 as observed by scanning electron microscope (SEM) and field emission scanning electron microscope (FESEM) indicated porous surface, whereas PFLSM as observed by SEM showed smooth topography. The inserted G6 in mice model showed satisfactory tissue growth with evidence of tissue compatibility as observed under Field emission scanning electron microscopy (FESEM).Histopathology studies of local tissue showed sufficient fibroblast and collagen growth indicating PFLSM-g-PAM-cl-MBA to be a promising pH-sensitive novel material for tissue proliferation as polymeric scaffold.