Abstract
Liposomal formulations have been used in the treatment of dermatological diseases with synthetic and herbal drugs incorporated to improve efficacy while herbal extracts reduce side effects associated with incorporated synthetic drugs. Anti-oxidants in Green Tea leaves and Roselle calyces were extracted into liposomes containing cholesterol by reverse phase evaporation. Varying molar ratios of Lecithin, Tween 80, Cholesterol at varying concentrations were analyzed and compared. The yield of the Green tea leaves water extracts (GTLW) and total Phenolic content (TPC) were found to be (30.35 ± 0.90%, 325.13 ± 1.04 mgGAE/g), Green tea leaves powder water extracts (GTPW) were found to be (27. 83 ± 1.38%, 272.65 ± 0.15 mgGAE/g). Roselle calyces water extracts (GHFW) were found to be (52.42 ± 3.23%, 37.82 ± 0.21 mgGAE/g), Roselle calyces powder water extracts (GHPW) were found to be (51.49 ± 1.30%, 50.00 ± 0.11 mgGAE/g). In the combination of water and organic solvent, the Green tea leaves extracts (GTLWC) were found to be (19.22 ± 1.20%, 246.67 ± 0.17 mgGAE/g), Green tea leaves powdered extracts (GTPWC) were found to be (19.42 ± 2.28%, 180.16 ± 0.81 mgGAE/g), Roselle calyces extracts (GHFWC) were found to be (52.42 ± 3.23%, 37.82 ± 0.21 mgGAE/g), Roselle calyces powdered (GHPWC) extracts were found to be (56.06 ± 1.9%, 38.52 ± 0.34 mgGAE/g). The ethanol extracts (GTLE & GTPE) are greenish with % average yield of 33.66 ± 0.23 & 34.86 ± 3.17. The water extracts (GTPW & GTLW) are light green in appearance with % average yield of 27.83 ± 1.38 & 30.35 ± 0.9. Ethyl acetate & chloroform (GTLWC & GTPWC) extracts shows brownish green in appearance with % average yield of 19.22 ± 1.20 & 19.42 ± 2.28 respectively. Phase contrast microscope (PCM) results show that ACT80E has particle size (PS) of (47.655 ± 10.32 <italic>μ</italic>m). Liposomes containing only cholesterol with Roselle extract (ACT80HB), with PS (54.588 ± 10.20). Liposomes containing only cholesterol with green tea extract (ACT80GT) is the highest with PS (69.668 ± 19.92). It was observed that the mean particle size of the green tea and Roselle extract loaded liposomes were higher than the unloaded liposomes
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