Preparation and characterization of Lecithin–heparin intercalated in montmorillonite nanocomposite

Abstract

Abstract In this work, a study of Lecithin–heparin intercalated in montmorillonite was performed in order to synthesize an antithrombogenic hybrid. The hybrid of montmorillonite (Mt)/Lecithin–heparin (LEC-HEP) was synthesized by the intercalation method under mechanical stirring. The composite was characterized by X-ray diffraction (XRD) analysis, X-ray fluorescence (XRF) and C, H, and N elemental analyses, zeta potentials, Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) techniques. The d001-value was increased by LEC-HEP intercalation. Fourier transform infrared spectroscopy also confirmed the intercalation of Lecithin–heparin into Mt interlayer space. In vitro release study of the antithrombogenic drug–Mt intercalates in phosphate buffer saline (pH 7.4) media at 37 °C was investigated. The heparin showed an initial burst effect for 24 h and then continuously released for 30 d. In-vitro blood compatibility of the Mt/LEC-HEP was carried out via hemolysis assay. The Mt/LEC-HEP exhibited hemolysis below 5% which is permissible for biomaterials. These modified materials have the potential for being used as blood contact materials.