Abstract
A home-made column (3.2 mm i.d., 100 mm length) was prepared in which glycidyl polymethacrylate was used. The optimum value of the initiator corresponded to 5 mg/mL. The column morphology was characterized by scanning electron microscopy. The permeability was evaluated using acetonitrile and water as a mobile phase and uracil as an unretained substrate. A simple and economical reversed-phase HPLC method has been developed for the simultaneous estimation of paracetamol and chlorzoxazone in their pharmaceutical formulations. Components were determined using a UV detector at 270 nm. The mobile phase was composed of 1% formic acid solution and acetonitrile (65/35, v/v); 0.7 mL/min flow rate and 5.0 µL injection volume were used. Peak resolution was 1.96. All findings allow concluding that the prepared stainless steel conventional HPLC column and the novel validated method are applicable for quality control and routine analysis.
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