Preparation and characterization of collagen-ciprofloxacin HCL membranes produced using gamma irradiation as a candidate for wound dressing

Abstract

Wound and bacterial infections are often found after periodontal surgery. Collagen has an important role in healing wounds. The purpose of this study is to produce a membrane film which has been antibacterial activity so that it can be developed as a candidate for periodontal wound dressing. Collagen from bovine tendons synthesized with the acidic method to obtain dry collagen. Dry collagen then dissolved to get a 2% collagen concentration and then mixed with ciprofloxacin HCl 0,1% (w/v) and poured on polyacrylic molds. The membrane is then irradiated by gamma rays irradiation at varying doses of 0, 15 and 25 kGy respectively. The irradiated membrane then characterized by its physicochemical properties in the form of functional group analysis, membrane pore size, water absorption, water vapor transmission, tensile strength and percent release of ciprofloxacin HCl. The result of the characterization is that the membranes didn’t show any significant changes in the functional group but show any significant changes in size membrane pores, water absorption, water vapor transmission rate, tensile strength, and percent release of ciprofloxacin hydrochloride after gamma rays irradiation.