Abstract
Soybean [Glycine max. (L.) Merr.] protoplasts have been permeabilized with dimethyl-sulfoxide, detergents and hypotonic shock as visualized by the fluorescent dye calcein. Maximum uptake was observed with hypotonic shock. Treated protoplasts were viable and underwent cell division in culture. The internalized dye was distributed throughout the cell, particularly in the vacuole. On the other hand, treatment of protoplasts with PEG resulted in a vesicular localization of fluorescence, concentrated in the cytoplasm. It was also shown that hypotonic shock results in protein (FITC: BSA) uptake.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
