Preparation and characterization of calcium phosphate bone cement with rapidly-generated tubular macroporous structure by incorporation of polysaccharide-based microstrips

Abstract

Calcium phosphate cements (CPCs) have been extensively used as bone graft substitutes for the repair of bone defect due to its biocompatibility, osteoconductivity and in-situ setting capability. They poorly degrade thus limiting their use in tissue engineering application. A possible strategy to improve the speed of CPC degradation is to add porogen to CPC to create macropores that can enhance cement resorption and can consequently be replaced by new bone. The as-generated macropores are generally not connected because of spherical shape of the porogens which can limit the extent of newly formed bone. The aim of this study was to fabricate CPCs having tubular macroporous structure by incorporating fast-dissolving maltodextrin microstrips (MDMS) and explore their properties such as setting time, mechanical property, microstructure and degradability of the cements. The results showed that after immersing MDMS-embedded composites in simulated body fluid under physiological condition for 1d MDMS rapidly disintegrated (more than 70%), generating tubular macropores in CPCs. The disintegration of MDMS completed in 1 week. CPCs containing MDMS lower than 30% by weight had the same final setting time as those without MDMS. The average values of compressive strength of the CPC composites decreased with the disintegration of MDMS. % Porosity and pore interconnectivity increased with increasing MDMS content. In addition, MDMS-embedded CPCs were cell friendly with excellent cell adhesion, indicating a possible candidate as bone graft substitutes.