Abstract
Optimal weight ratios for the preparation of lactoferrin (LF), N-succinyl chitosan (SCh) and galactomannan (GM)-based polyelectrolyte complexes (PEC) were determined. Soluble PEC were obtained using solutions of biopolymers: lactoferrin (2mg/ml), N-succinyl chitosan (8mg/ml) and galactomannan (16mg/ml) in 0.15M NaCl, pH6.5±0.1, under normal conditions at a weight ratio of 1:1 1:2 and 1:3 LF:GM; 1:1, 1:2, 1:3 and 1:4 LF:SCh; 1:3:2 and 1:2:3 LF:SCh:GM. Using the method of heat treatment below the temperature of protein denaturation from the soluble complexes were formed stable biopolymer particles which have size characteristics of 250–400nm, regular spherical shape, a negative zeta potential of 12–17mV and good storage stability. The load of lactoferrin in the particles was 76–87%. Yield of freeze dried particles was 70–80%. The obtained particles could eventually be used for the targeted transport of biologically active substances.
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