Preparation And Characterization of Bio-\\Polymeric Nano Feed Incorporating Silage-Derived Organic -Acids And The Polar Fraction of Papaya Leaf Extract

Abstract

This research has aimed to incorporate the fermented organic acids and papaya leaf extract into chitosan-alginate composites using an ionic -gelation method. The concentration of chitosan was 35 mg/7.5 mL, sodium alginate was 20 mg/7.5 mL, and cross-linking the CaCl2 cross linker was 20 mM. Variations in the formula Different formulations, are carried out on organic acids with the code of referred to as kumpai-ZnO, legum-ZnO, and kumpai-legum-ZnO. These sample using various organic acids, and the formulations contained as much as 50 mg/7.5 mL of the organic acid. The results of the identification of the chemical contents of the fermented product was lactic acid derivatives as a major component, and there were no alkaloids, flavonoids, terpenoids, and or poly phenols content present. The particle characterization results showed they were a homogeneously distributed in size with PDIs from 0.199 to 0.218 with a sizes between of 253.6 to and 286.8 nm. The potential Zeta potential is was found to be -20 to -15 mV by using a particles size analyzer (PSA). Particles were spherical based on the images of transmission electron microscopy (TEM) images. The best formula formulation was is the formula 3, containing with the kumpai-legum-ZnO content, which was demonstrated by to be 85% encapsulation percentage encapsulated (%EE). The anti-E. coli activity test showed an increase of the particles were 40% of activity the particles compare to more active than the same substances not in particle. The ionic -gelation method successfully created a homogenous particle suspension loading loaded with the active compounds, such as the fermented organic acids and the papaya leaf extract. This system could deliver the active compounds to the bacterial cell of E. coli and S. typimurium bacterial cells.