Abstract
The use of microspheres nowadays is important in the pharmaceuticals formulation. The aim of the present research was to evaluate physical characteristics of ovalbumin-loaded alginate microspheres using aerosolisation technique at various concentrations of alginate and BaCl 2 . Alginate microspheres were characterized in terms of encapsulation efficiency, yield, particle size, surface morphology and protein integrity. The comparative study between concentrations of BaCl 2 and alginate polymer was investigated. Increasing concentration of alginate polymer from 2.5 to 3.5% reduced their particle size and formed smoother and spherical microspheres. Increasing Ba 2+ concentration, simultaneous increased the encapsulation efficiency and yield. Scanning electron microscope (SEM) photomicrographs revealed that with the increase in the electrolyte concentration the density of the cross link is also increased. Smoother surface was demonstrated when the electrolyte concentration is increased from 0.5M to 0.75M. Formula prepared using 0.75M BaCl2 and 3.5% alginate polymer resulted the highest encapsulation efficiency (92%), the highest microspheres yield (73%) and the smallest microspheres size (3.73µm). Most of the formulations maintain the integrity of ovalbumin after 2 hours incubation in pH 1.2 followed by 8 hours incubation in pH 7.4. The different formulations produced different physical characteristics of ovalbumin-loaded alginate microspheres.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.