Abstract
Inflammation leads to chondrocyte senescence and cartilage degeneration, resulting in osteoarthritis (OA). Adipose‐derived stem cells (ADSCs) exert paracrine effects protecting chondrocytes from degenerative changes. However, the lack of optimum delivery systems for ADSCs limits its use in the clinic. The use of extracellular matrix based injectable hydrogels has gained increased attention due to their unique properties. In the present study, we developed hydrogels from amnion tissue as a delivery system for ADSCs. We investigated the potential of amnion hydrogel to maintain ADSC functions, the synergistic effect of AM with ADSC in preventing the catabolic responses of inflammation in stimulated chondrocytes. We also investigated the role of Wnt/β-catenin signaling pathway in IL-1β induced inflammation in chondrocytes and the ability of AM-ADSC to inhibit Wnt/β-catenin signaling. Our results showed that AM hydrogels supported cell viability, proliferation, and stemness. ADSCs, AM hydrogels and AM-ADSCs inhibited the catabolic responses of IL-1β and inhibited the Wnt/β-catenin signaling pathway, indicating possible involvement of Wnt/β-catenin signaling pathways in IL-1β induced inflammation. The results also showed that the synergistic effect of AM-ADSCs was more pronounced in preventing catabolic responses in activated chondrocytes. In conclusion, we showed that AM hydrogels can be used as a potential carrier for ADSCs, and can be developed as a potential therapeutic agent for treating OA.
Highlights
Inflammation leads to chondrocyte senescence and cartilage degeneration, resulting in osteoarthritis (OA)
amnion membrane (AM) tissue using a simple alkaline lysis method and the decellularization process was evaluated by propidium iodide (PI) staining to detect residual DNA on the AM tissue
PI staining of decellularized AM tissue samples showed no detectable DNA residues (Fig. 1B)
Summary
Inflammation leads to chondrocyte senescence and cartilage degeneration, resulting in osteoarthritis (OA). We developed hydrogels from amnion tissue as a delivery system for ADSCs. We investigated the potential of amnion hydrogel to maintain ADSC functions, the synergistic effect of AM with ADSC in preventing the catabolic responses of inflammation in stimulated chondrocytes. We developed an amnion membrane (AM) hydrogel with the hypothesis that the AM hydrogel would retain ADSCs; maintain their viability, proliferation, and stemness, further modulating the catabolic responses (inflammation, expression of matrix degrading enzymes) in activated chondrocytes synergistically with ADSCs. The present study, aimed to develop an injectable AM based hydrogel for ADSC delivery, which may together prevent the catabolic responses in IL-1β activated chondrocytes. We investigated the role of Wnt/β-catenin signaling which play a major role in transducing inflammatory and catabolic signals in joint degeneration
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
