Preparation and Characterization of Agarose-Gelatin Blend Hydrogels as a Cell Encapsulation Matrix: An In-Vitro Study

Abstract

Cell encapsulation represents an alternative nonviral technique to treat multiple diseases, leading to a reduction or even absence of administration of immunosuppressants. Hydrogels have been introduced as novel materials suitable for cell encapsulation. This study involves agarose–gelatin blend hydrogels with four different weight percentage ratios (100:0, 75:25, 50:50, 25:75) of agarose to gelatin. Prepared blend hydrogels were assessed in terms of rheological behavior (gel point by using complex viscosity), cell attachment (hemocytometer), cell viability and cytotoxicity (3-(3,4-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliium bromide, MTT assay), and mechanical and integral stability (Bradford test and shear force rupture assay, respectively). Based on the obtained rheological experimental results, the sol-gel transition point for 50:50 was in the physiological condition range (35°C–37°C). The percent of nonattached cells on the surface of the hydrogel decreased from 92% for the 100:0 sample to 46.3% for the 50:50 sample, and the cell viability was more than 95%. A good structural integrity was achieved for samples with weight ratio of 50:50; 20.195% gelatin was released during the 24 h in phosphate buffer solution at 25°C and the mechanical stability of agarose–gelatin microcapsules under shear force were improved about 14% rather than pure agarose microcapsule.