Abstract
It is shown that trichloro-1,3,5-triazine can be attached to aminated silica gel at two sites. This method of activating the silica gel for immobilization of analytical reagents is considered in relation to the typical single-site method of attachment. Diffuse-reflectance infrared and ultraviolet spectroscopic results are presented to confirm the nature of the attachment. 2,2′,4-Trihydroxyazobenzene is used as a model reagent to illustrate that better control of the immobilization reaction results from the second site of attachment of the triazine linkage to the substrate.
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