Preparation and characterization analysis of molecular contrast agent gold nanoparticle-recombinant high density lipoprotein

Abstract

Objective To produce the molecular contrast agent gold nanoparticle-recombinant high density lipoprotein(Au-rHDL), using gold nanoparticle(AuNP)and recombinant high density lipoprotein(rHDL)as raw materials, and to analyze the characterization of the agent. Methods The AuNP was prepared using chemical reduction method, and it interacted with rHDL to synthesize Au-rHDL complex.The comlex was analyzed and detected using uv-visible absorption spectrum(UV-vis)and transmission electron microscope(TEM). The X-ray attenuation and extracorporeal CT imaging of the complex were compared with the conventional iodine contrast agent Omnipaque. Results Au-rHDL had excellent character of dispersion and uniformity, and the particle size of the complex was 12 nm.The Au-rHDL complex had a higher attenuation of X-ray and a better effect of extracorporeal CT imaging, compared with Omnipaque. Conclusion The molecular contrast agent Au-rHDL is prepared successfully, which lays the foundation for the molecular targeted imaging of atherosclerotic plaque. Key words: Recombinant high density lipoprotein; Gold nanoparticle; Molecular contrast agent; Atherosclerosis