Preparation and Biomineralization of Injectable Hydrogel Composite Based Chitosan-tetronic and BCP Nanoparticles

Abstract

In this study, a hydrogel composite was enzymatically prepared with rapid gelation time from tyramine-tetronic-conjugated chitosan and biphasic calcium phosphate (BCP) nanoparticles. Compressive stress of the hydrogel composite was reached at 591±20 KPa with 10 wt% of loaded BCP. Degradation study of the material showed 20% of weight loss after 4 weeks. In vitro study with MG 63 osteoblast cell evidenced that the cells were well-attached on hydrogel composite surfaces. Biomineralization on the hydrogel composites surfaces was well-observed after soaking for 14 days in simulated body fluid (SBF) solution. The obtained results indicated that the hydrogel composite could be an injectable potential material for bone regeneration.