Abstract
A quinine-functionalized poly(1-vinylimidazole)-modified hydrophilic silica stationary phase (Sil-PIm-Qn) was prepared by surface radical chain transfer and nucleophilic substitution reactions. Sil-PIm-Qn was characterized by elemental analysis and FT-IR. The chromatographic characteristics of the stationary phase were investigated in the hydrophilic interaction chromatographic (HILIC) mode. The results showed that five amino acids, nine sulfonamides, and ten nucleosides/nucleobases could be successfully separated on this stationary phase, respectively. The retention behavior of the compounds on the column was evaluated under different chromatographic conditions, i. e., for different salt concentrations and acetonitrile contents in the mobile phases. The stability of this column was also investigated, and the relative standard deviation (RSD, n=10) of the retention time of Sil-PIm-Qn was 0.08%-2.30%. Given its simple and green preparation as well as its unique selectivity and high separation efficiency, Sil-PIm-Qn is expected to find widespread application in the separation of hydrophilic compounds, including sulfonamides and nucleosides/nucleobases, in real samples.
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