Abstract
Porous cross-linked poly (glycidyl methacrylate-divinylbenzene) (PGMA-DVB) particles (7.42 ± 0.24 μm in diameter) were prepared by an improved two-step seed swelling polymerization method. The PGMA-DVB particles were further modified with poly (allylamine hydrochloride) (PAH) and 4-(bromomethyl) phenylboronic acid (BPA), which were used as high performance liquid chromatography (HPLC) filler for nucleotides separation. The liquid chromatographic column packing materials successfully achieved complete separation of nucleotides mixture or deoxynucleotides mixture based on non-polar adsorption, hydrogen bonding interaction and electrostatic adsorption. Reckoning on the chemical structure of BPA, nucleotides and deoxynucleotides with same base group were also separated. The column packing materials were durable after over 100 time running or 7 days. It presents a kind of new notion for the separation of nucleotides by HPLC.
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