Abstract
A new liquid chromatographic ligand exchange CSP has been prepared by covalently bonding ( S)- N, N-carboxymethyl undecyl leucinol monosodium salt onto silica gel and employed in resolving various α-amino acids. The new CSP was quite good in resolving various α-amino acids and the resolution results were dependent on the type and content of organic modifier in the mobile phase. From these results, a chiral recognition model using a lipophilic interaction between the tethering alkyl group of the CSP and the substituent at the chiral center of α-amino acids was proposed. The liquid chromatographic resolution of α-amino acids on the new CSP was also found to be dependent on the Cu(II) concentration in the mobile phase and the column temperature.
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