Abstract
In order to face the increasing demand of laccase with high stability and high performance, laccase was chemically modified by acid anhydride, and copper ion (Ⅱ) was coordinated with the hetero atom of the modified laccase to obtain the nanoflower biocatalyst. The effects of different reaction time, laccase concentration and copper sulfate concentration on the formation of nanoflowers were investigated in detail. Compared to free laccase, the enzyme activity, pH stability and thermal stability of nanoflowers increased by 165%, 375% and 430%, respectively. Furthermore, the catalytic properties of our nanoflower biocatalysts were investigated by catalyzing the synthesis of viniferin. The results showed that a higher yield of 45.0% was obtained in shorter period of time than that of the free laccase (21.6%), and the product was single without other by-products. What’s more, nanoflower biocatalyst exhibited excellent storage stability, repeatability and reproducibility in cycle operation. We believed that this study provided a feasible and efficient biocatalytic method for conversion of resveratrol to viniferin. These preliminary results indicated that nanoflowers would have great potential for application in biotransformation and biocatalysis.
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