Abstract
Mitochondria from green leaves of spinach ( Spinacia oleracea L) have been prepared by a preparation procedure using differential centrifugation and partition in an aqueous dextran-poly(ethylene glycol) two-phase system. The enrichment of mitochondria in relation to chloroplast material was 200–400 times based on NAD: isocitrate dehydrogenase (IDH) activity and chlorophyll content. The protein/chlorophyll ration in the preparation was approximately 100. The apparent intactness of both the inner and the outer membrane was about 90%, as measured by latency of enzyme activities. The mitochondria oxidized NADH (respiratory control 2.1), succinate (1.6), glycine (3.8) and malate (3.0). The mitochondrial preparation was morphologically characterized by electron microscopy.
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