Preovulatory Follicular and Subsequent Luteal Size Influence Pregnancy Success in Water Buffaloes

Abstract

The diameter of the preovulatory follicle (POF) and its effects on subsequent corpus luteum (CL) size and conception were studied in 38 lactating indigenous cycling buffaloes in the Mymensingh district of Bangladesh. Body condition score (BCS) at estrus was estimated for the buffaloes. The buffaloes were synchronized with two injections of a synthetic analogue of PGF2α administered 11 days apart. Transrectal ultrasonography was carried out at estrus and on days 5, 9, 12 and 16 post ovulation to determine the POF and successive CL size. Pregnancy was confirmed by ultrasound examination on day 40-45 post ovulation. Twenty one (55.3%) buffaloes were diagnosed as pregnant. The conception rates of thin (BCS ≤2.0), good (BCS 2.5-3.5) and fat (BCS glt;3.5) buffaloes were 7.7, 88.2 and 62.5% (χ² = 19.54; P<0.05), respectively. The mean diameter of the POF at estrus was larger (P<0.01) in buffaloes that ultimately were diagnosed as pregnant compared with their nonpregnant counterparts (13.7 ± 0.3 vs. 11.2 ± 0.5 mm, respectively). The conception rates of buffaloes having small (9 to ≤ 12 mm), medium (>12 to ≤14 mm) and large (>14 to 16 mm) POFs at estrus were 9.1, 70.0 and 85.7% (χ² = 13.87, P<0.01), respectively. On day 5 post ovulation, CL size was positively correlated (CL: r=.74, P<0.01) with POF diameter. Retrospective analysis revealed that on day 5 post ovulation, the pregnant buffaloes had higher (P<0.01) post ovulation CL sizes than their nonpregnant counterparts (15.6 vs. 11.8 mm). Similarly, on day 9 post ovulation, the difference in CL size (14.3 vs. 13.6 mm) between pregnant and nonpregnant buffaloes was significant (P<0.05). In conclusion, the diameter of the POF in buffaloes has a positive impact on the size of the post ovulation CL and conception.