Prenatal oxidative stress: I. Malondialdehyde in hypoxic and hyperoxic chick embryos

Abstract

Evidence suggests a positive correlation between metabolic rate (Vo 2), or ambient oxygen (O 2) tension, and the rate of formation of free radicals from O 2. We have previously demonstrated that the rates of growth, Vo 2, protein and DNA accumulation, and the activity of cytochrome oxidase (a key mitochondrial respiratory enzyme), are increased significantly by exposing the chick embryo to 72 h of hyperoxia (60% O 2) late in incubation. To test the hypothesis that the chick embryo responds to a prenatal alteration in O 2 availability in such a way as to protect its tissues from oxidative damage, we have used the thiobarbituric acid assay to estimate lipid peroxidation (a major form of free radical damage) in selected organs from chick embryos exposed to altered O 2 availability. We found significantly higher concentrations of malondialdehyde (MDA, a secondary product of lipid peroxidation) in liver than in chorioallantoic membrane, brain, or heart. However, embryos exposed to brief (72 h) hypoxia (15% O 2) or hyperoxia (60% O 2) late in incubation, or 48 h of such exposure followed by 24 h of incubation in pure O 2, exhibited no significant difference in MDA levels compared to normoxic (21% O 2) controls in any of the tissues examined. We conclude that the increase in aerobic metabolism induced in the chick embryo by 3 days of hyperoxia is not accompanied by an increase in lipid peroxidation. We postulate that the chick embryo adapts to hyperoxia in such a way as to escape additional free radical damage, perhaps by increasing the capacity of its antioxidant defenses to compensate for a potential increase in the rate of free radical generation.