Prenatal exposure to hesperidin improves reflexive motor behaviors in mice offspring.

Abstract

Prenatal exposure during the embryonic period has positive or adverse effect on newborn brain development. Neuroprotective activity of the hesperidin is well documented but there is no evidence for maternal exposure to hesperidin on offspring reflexive motor behaviors. So, the aim of the current study was to determine the prenatal exposure to hesperidin on reflexive motor behaviors in mice offspring. Forty pregnant female NMRI mice (8-10weeks old) were allocated into four groups. Group 1 kept as control and groups 2-4 intraperitoneal (i.p) injected with hesperidin (0.1, 0.5, and 1mg/kg) on days of 5, 8, 11, 14, and 17 of pregnancy. The control group injected with saline at the same days. Following delivery, 20 pups from each litter were selected and reflexive motor behaviors determined using ambulation, hind-limb foot angle, surface righting, hind-limb strength, grip strength, front-limb suspension, and negative geotaxis tests. At the end of the study serum Malondialdehyde (MDA), Superoxide dismutase (SOD), Glutathione peroxidase (GPx), and total antioxidant status (TAS) levels were determined. According to the results, maternal exposure to hesperidin (0.1, 0.5, and 1mg/kg) increased ambulation score, front-limb suspension time, and hind-limb suspension score in mice offspring compared to the control group (p<.05). Hesperidin (0.1, 0.5, and 1mg/kg) decreased hind-limb foot angle in mice offspring compared to the control group (p<.05). Prenatal exposure to hesperidin (0.5 and 1mg/kg) significantly increased the surface righting and grip strength in comparison to the control group (p<.05). Hesperidin (0.1, 0.5, and 1mg/kg) decreased MDA and increased SOD and GPx levels in mice offspring (p<.05). These results suggested hesperidin exposure during pregnancy has positive effect on reflexive motor behaviors in mice offspring may be due to its antioxidant activity.