Prenatal and Postnatal Cigarette Smoke Exposure Is Associated With Increased Risk of Exacerbated Allergic Airway Immune Responses: A Preclinical Mouse Model

Ingrid Van Ark,Jeroen Van Bergenhenegouwen,Johan Garssen,Marthe T Van Daal,Saskia Braber,Gert Folkerts,Thea Leusink-Muis,Hamed Janbazacyabar

doi:10.3389/fimmu.2021.797376

Abstract

Increased exposure to household air pollution and ambient air pollution has become one of the world’s major environmental health threats. In developing and developed countries, environmental cigarette smoke (CS) exposure is one of the main sources of household air pollution (HAP). Moreover, results from different epidemiological and experimental studies indicate that there is a strong association between HAP, specifically CS exposure, and the development of allergic diseases that often persists into later life. Here, we investigated the impact of prenatal and postnatal CS exposure on offspring susceptibility to the development of allergic airway responses by using a preclinical mouse model. Pregnant BALB/c mice were exposed to either CS or air during pregnancy and lactation and in order to induce allergic asthma the offspring were sensitized and challenged with house dust mite (HDM). Decreased lung function parameters, like dynamic compliance and pleural pressure, were observed in PBS-treated offspring born to CS-exposed mothers compared to offspring from air-exposed mothers. Maternal CS exposure significantly increased the HDM-induced airway eosinophilia and neutrophilia in the offspring. Prenatal and postnatal CS exposure increased the frequency of Th2 cells in the lungs of HDM-treated offspring compared to offspring born to air-exposed mothers. Offspring born to CS-exposed mothers showed increased levels of IL-4, IL-5 and IL-13 in bronchoalveolar lavage fluid compared to offspring from air-exposed mothers. Ex-vivo restimulation of lung cells isolated from HDM-treated offspring born to CS-exposed mothers also resulted in increased IL-4 production. Finally, serum immunoglobulins levels of HDM-specific IgE and HDM-specific IgG1 were significantly increased upon a HDM challenge in offspring born to CS-exposed mothers compared to offspring from air-exposed mothers. In summary, our results reveal a biological plausibility for the epidemiological studies indicating that prenatal and postnatal CS exposure increases the susceptibility of offspring to allergic immune responses.

Highlights

According to the World health Organization (WHO), approximately 300 million people currently suffer from asthma [1], and it is estimated that the global prevalence of asthma increases by 50% every decade with the most striking increase among children [2, 3]
Substantial evidence indicates that household air pollution (HAP) and ambient air pollution (AAP) is associated with enhanced prevalence of different types of pollution-related diseases, including allergic asthma [32, 42,43,44,45]
Results from different epidemiological and experimental studies indicate that there is a strong association between maternal HAP, cigarette smoke (CS) exposure, and the development of allergic diseases that often persists into later life [47,48,49,50]

Summary

Introduction

According to the World health Organization (WHO), approximately 300 million people currently suffer from asthma [1], and it is estimated that the global prevalence of asthma increases by 50% every decade with the most striking increase among children [2, 3]. The rapid rise in asthma and allergy incidence throughout the world implies that, in addition to hereditary factors, environmental factors, including air pollution, may play a role [4, 5]. Several factors participate in the development of asthma, emerging evidence from several epidemiological and experimental studies indicate that maternal exposure to environmental factors, such as air pollution and cigarette smoke (CS) exposure may contribute to the development of childhood asthma [7,8,9,10,11,12,13]. Early life exposure to a variety of environmental factors, including air pollution and CS exposure, can predispose neonates to diseases and affect the dynamics of immune responses in later life [27, 28]. Numerous studies have indicated that fine/ultrafine PM can cross epithelial barriers, including the placental barrier, suggesting that exposure to environmental contaminants might already take place in the womb [29,30,31]

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