Prematür Overyan Yetmezlikli Ratlarda Yaş Kupa Terapisinin Anksiyete ve Tiyol/Disülfit Homeostazı Üzerine Etkileri: Deneysel Bir Çalışma

Abstract

Objective: The effect of wet cupping therapy on anxiety and thiol/disulfide homeostasis was evaluated in rats with premature ovarian failure (POF) induced by cyclophosphamide (CYC). Material and Methods: Twenty four Wistar Albino female rats were divided into control (n=6), POF (n=6), wet cupping (n=6), POF+wet cupping (n=6) groups. CYC at a dose of 200 mg/kg was injected intraperitoneally to POF and POF+wet cupping groups. One week later, wet cupping therapy was applied to wet cupping and POF+wet cupping groups for 5 minutes. The next day, Open Field Test and Elevated Plus Maze Test were applied to all experimental groups to evaluate anxiety. After sacrification, to assess pattern formation, endometrial thickness and ovarian reserve were measured with hematoxylin-eosin staining. Thiol/disulfide homeostasis and erythrocyte oxidized-reduced glutathione levels were measured in serum to measure oxidative stress. Results: Although there was no statistical significance, it was observed that anxiety decreased in POF+wet cupping compared to POF group. There was no change in thiol-disulfide values in POF+wet cupping compared to POF group (p>0.05). Conclusion: Wet cupping therapy did not reduce anxiety in the experimental POF model and did not shift the balance to positive in thiol/disulfide homeostasis in favor of thiols. However, the obtained graphics, numerical data and high standard deviation suggest that meaningful data could be obtained with more subjects.