Preliminary toxicity and thin layer chromatographic studies of Pteleopsis habeensis leaves

Abstract

Oral acute toxicity test in which a single dose is used in an animal on one occasion is to identify the gross behaviour and the dose that will cause the animal to die. To investigate the phytoconstituents and toxicity of different extracts of P. habeensis in wistar strain Albino rats. The acute toxicity was determined through oral administration of different doses of P. haeensis leaf extracts to Wistar albino rats in increasing concentrations to ascertain its safety. The animals were monitored daily for 10 days for signs of toxicity such changes in their general behaviour and death as endpoint. The lethal dose (LD50) of P. habeensis leaves extract were found to be ≥ 4000 mg/kgand there was no any sign of toxicity or changes in the gross behaviour when observed. The extracts were found to contain some important phytochemical constituent such as alkaloid, flavonoids, Tannin and Triterpenoids. TLC studies illustrated the spots of different phytoconstituent presents. The findings revealed that with the different extracts of P. habeensis no sign of toxicity on short term exposure of a single dose and contains different active constituent which are associated with some pharmacological effects.