Abstract
Agrobacterium rhizogenes-transformed hairy roots from Catharanthus roseus have been widely used in research and in life. Consequently, methods for preservation are essential to maintain valuable hairy root lines. Our results showed that Gamborg’B5 was the most suitable medium for hairy roots from VIN002 and VIN005 while solid White media was more comfortable for the hairy lines from VIN022 and VIN077. All hairy root lines must be preserved in the dark at 25–27 oC. Under suitable conditions, the rate of lines growing normally reached 69.3, 67.0, 57.3 and 60.7 % for VIN002, VIN005, VIN072, and VIN077, respectively. There were 90.0, 93.3, 80.0 and 93.3 % of lines could preserve rolB, a gene which has important effect on the growth of hairy roots.
Highlights
Ahmad, Identification of new compounds establishment of adventitious and hairy root from Catharanthus roseus hairy root cultures, cultures of Plumbago zeylanica L., African
Our results showed that Gamborg’B5 was the most suitable medium for hairy roots from VIN002 and VIN005 while solid White media was more comfortable for the hairy lines from VIN022 and VIN077
Don hairy root lines induced by Agrobacterium rhizogenes isolated in Vietnam x Nguyen Nhu Nhut University of Science, VNU-HCM Giatuong Company Limited, Binhduong branch x Bui Van Le University of Science, VNU-HCM
Summary
Rễ tơ cây Dừa cạn thu được từ việc chuyển gene nhờ Agrobacterium rhizogenes được ứng dụng nhiều trong nghiên cứu và đời sống. Rễ tơ từ cây Dừa cạn cũng đang được quan tâm nghiên cứu do chúng có khả năng tổng hợp nhiều hợp chất thứ cấp có giá trị [14]. Đa số các dòng rễ tơ nói chung được nuôi cấy bảo quản trên môi trường thạch hoặc môi trường lỏng [20]. Cho đến nay, rễ tơ cây Dừa cạn cũng được bảo quản chủ yếu bằng phương pháp cấy chuyền trên môi trường thạch hoặc môi trường lỏng. Rễ tơ cây Dừa cạn được nuôi cấy trên môi trường thạch ở các điều kiện khác nhau như môi trường cơ bản để nuôi cấy, ánh sáng và nhiệt độ để chọn lọc điều kiện bảo quản thích hợp
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