Abstract
Background. Significances of CD133 mRNA in peripheral blood mononuclear cells (PBMCs) of gastric adenocarcinoma (GC) patients were investigated. Methods. Correlations of CD133 mRNA expression in PBMCs on clinicopathological parameters or CD133 protein expression were analyzed. Receiver operating characteristic curve according to bright scale value (BSV) of CD133 mRNA was used to group patients for prognosis analysis. Results. BSV of preoperative CD133 mRNA in PBMCs in GC was significantly higher than that in volunteers or in GU. Invasive depth or metastatic lymph node number for higher BSV of preoperative CD133 mRNA and invasive depth or lymphatic vessel invasion for higher BSV of postoperative CD133 mRNA in the PBMCs were identified. Patients with CD133+ expression in primary lesion had a significantly higher expression of preoperative CD133 mRNA in the PBMCs. The expression of preoperative or postoperative CD133 mRNA in PBMCs related positively to CD133 mRNA expression in primary lesion. Patients with higher expression of preoperative or postoperative CD133 mRNA shared significantly shorter survival compared with that in lower expression group. Conclusion. Higher levels of preoperative or postoperative CD133 mRNA in PBMCs of GC correlated positively to the lymphatic metastasis and the BSV of CD133 mRNA in primary lesion, indicating the poorer survival.
Highlights
Significances of CD133 mRNA in peripheral blood mononuclear cells (PBMCs) of gastric adenocarcinoma (GC) patients were investigated
By two independent samples tests of nonparameter of statistic analysis, it was found that the average bright scale value (BSV) of CD133 mRNA in the group of patients with GC was significantly higher than that in the group of volunteers (Z = −4.132, P < 0.001) or in the group of patients with gastric ulcer (GU) (Z = −3.688, P < 0.001), respectively, but there was no statistic difference between the group of volunteers and the group of patients with GU (Z = −0.695, P = 0.487)
The above-mentioned results indicated that the measure of CD133 mRNA from PBMCs could be worth of further investigations as a diagnostic value in GC if combined with the examination of gastric fiber or radiological imaging observation
Summary
Significances of CD133 mRNA in peripheral blood mononuclear cells (PBMCs) of gastric adenocarcinoma (GC) patients were investigated. Gastric cancer (GC) with only about 20% of overall 5-year survival rate until now is one of the most common forms of cancers worldwide because it is usually diagnosed too late, especially in the developing countries such as China, due to defections of typical symptoms, effectively imaging means and specific tumor biomarkers Such advanced cases with later stages of TNM are often unsuitable to receive a curative surgery [1]. A lot of studies suggested that a small population of cancerous cells named as socalled cancer stem cells (CSCs) with unique self-renewal properties and malignant potentials existed in some sorts of solid tumors [2,3,4] As reported, these CSCs could be sorted by flow cytometry or by immunomagnetic separation (IMS) and had been demonstrated to share the stronger abilities of tumorigenicity in vivo and cloning sphere formation in vitro [3,4,5]. Some investigations found that, in patients with some advanced malignancy, the high level of CEPs in the peripheral blood
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