Preliminary study on the differences of fucosyltransferase 2 gene polymorphism between Uyghur and Han patients with ulcerative colitis

Abstract

Objective To preliminarily evaluate the differences of fucosyltransferase 2 (FUT2) gene polymorphism between Han and Uyghur patients with ulcerative colitis (UC) in Xinjiang Uyghur Autonomous Region.Methods A total of 120 UC patients were enrolled,of whom 64 patients were Han and 56 patients were Uyghur.And there were another 320 healthy controls with matched age,gender and nationality.The DNA of subjects was extracted and the polymorphism of gene FUT2 at C357T,A385T,G428A,and G739A was detected by polymerase chain reaction (PCR) and gene sequencing.The chi-square test was performed to compare gene allele frequencies between Han,Uyghur UC patients and healthy controls.Results The gene frequencies of CC,CT,and TT in C357T mutation of Han UC patients were 0,11.3％ and 88.7％,respectively,while in the corresponding healthy controls were 5.5％,26.1 ％％ and 68.4％,respectively (x2 =9.091,P =0.011).The gene frequencies of AA,AT and TT in A385T mutation of Uyghur UC patients were 87.8％,8.2％ and 4.0％,respectively,while in the corresponding healthy controls were 59.7％,33.6％ and 6.7％,respectively (x2 =13.480,P=0.010).The gene frequencies of GG,AG and AA inG428A mutation of Han UC patients were 98.1％,1.9％ and 0,respectively,while in the corresponding healthy controls were 83.9％,14.3％ and 1.8％,respectively (x2=7.382,P=0.025).The gene frequencies of GG,AG and AA in G428A mutation of Uyghur UC patients were 69.4％,18.4％ and 12.2％,respectively,and in healthy controls were 35.6％,51.0％ and 13.4％,respectively (x2 18.790,P＜0.01).There were no significant differences in G739A mutation between Han and Uyghur UC patients and healthy controls (all P＞0.05).Conclusions The FUT2 gene polymorphisms are associated with UC.C357T and G428A are associated with UC in Han population.A385T and G428A are in the Uyghur population,which indicates gene FUT2 may play a potential role in the pathogenesis of UC in Xinjiang region. Key words: Colitis, ulcerative; Fucosyltransferases; Polymorphism, genetic; Xinjiang