Abstract
This study examined the effect of two different extenders (carbohydrate and mineral based), supplemented with 10% Dimethyl sulfoxide (DMSO), on cryopreservation of brown trout (Salmo trutta macrostigma) sperm used for hybridization with rainbow trout (Oncorhunchus mykiss). The carbohydrate based extender resulted in higher fertilization (42.5±1.4%) than the mineral based extender and there was no difference in fertilization rates (p>0.05). This study successfully demonstrated that it is possible to produce Salmo trutta macrostigma x Oncorhynchus mykiss hybrids using cryopreserved sperm from brown trout.
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