Abstract
Our aim was to validate the effectiveness of steep pulse irreversible electroporation technology in human large cell lung cancer cells and to screen the optimal treatment of parameters for human large cell lung cancer cells. Three different sets of steep pulse therapy parameters were applied on the lung cancer cell line L9981. The cell line L9981 inhibition rate and proliferation capacity were detected by Vi-Cell vitality analysis and MTT. Steep pulsed irreversible electroporation technology for large cell lung cancer L9981 presents killing effects with various therapy parameters. The optimal treatment parameters are at a voltage amplitude of 2000V/cm, pulse width of 100μs, pulse frequency of 1 Hz, pulse number 10. With this group of parameters, steep pulse could have the best tumor cell-killing effects.
Highlights
A steep pulse can cause unique intracellular effects that induce irreversible electrical breakdown, effectively killing tumor cells
Previous studies have shown that a steep pulse has a role in killing different types of tumor cells that has not been extensively reported in the literature (Luo et al, 2012)
Research will verify the validity of steep pulse in killing the lung cancer cell line L9981
Summary
A steep pulse can cause unique intracellular effects that induce irreversible electrical breakdown, effectively killing tumor cells. Steep pulse can suppress tumor growth and proliferation. It shows a good application prospect in comprehensive treatment of the tumor (Luo et al, 2012). It has opened up a new method for the study and control of biological cells. We screened the best treatment parameters for L9981, and provide a theoretical basis for researching the mechanism of action of steep pulsed and irreversible electrical breakdown
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