Abstract
Liquid waste from boiling process of batik industry contains toxic compounds including wax, dye, soda ash, metal, cloth etc. If it is disposed into the environment without any treatment it will pollute the environment and threaten lives. Several methods to treat liquid waste of batik industry have been implemented, including by physical, chemical, and biological processes. The study to obtain potential bacteria in decolorization of liquid waste from boiling process of batik industry has been initiated. Bacteria were isolated from ditch soil around local batik industry in Medan. Fifteen bacterial isolates were obtained. Screening to obtain potential isolates to decolorize waste was done by culturing the isolates in solid minimal salt media containing increases concentration of liquid waste starting from 25%, 50% to 75%, no any other carbon source added to the media. Six isolates showed the growth in media containing 75% waste. All six isolates were then tested for the growth in minimal salt medium broth containing 75% of waste. Three isolates SP2, SP3, and SP4 exhibited good growth at this waste level media. These isolates were considered as potential bacteria and are subjected to be used for further decolorization analyses. Molecular identification of isolates based on 16S rRNA is also in process.
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