Abstract
Objective To isolate dental pulp stem cells(DPSCs)from human dental pulp cells (HDPCs)in vitro and examine their potential tO differentiate into osteoblast-like cells.Methods HDPCs were separated and cultured from dental pulp of youngman healthy premolars extracted for orthodontic purpose.Vimentin and cytokeratin expression were examined by immunocytochemical staining.DPSCs were isolated from cultured HDPCs by magnetic-activated cell sorting,S(MACS)indirect magnetic celllabeling and positive selection strategy with antibody Stm-1,and then they were induced to differentiation by mineralizing culture medium.The expression of Stro-1 and Alkaline phosphatase(ALP)were evaluated before and after induction.Resuits The cultured cells appeared to be fibroblast-like cells,staining positive for vimentin and negative for cytokeratin.The rate of human dental pulp-derived Stro-1+cells was about 10%.DPSCs demonstrated no expression of Stro-1 but strong expression of ALP after induced differentiation with conditional medium.Conclusions MACS may be the effective way to isolate DPSCs,which with differentiation potential. Key words: Dental pulp stem cells; MACS; Induced differentiation
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