Abstract
to verify the effectiveness of coconut water in preserving tissues for transplant. Fifty male Wistar rats were randomly distributed in five groups, according to the following preservation solutions for tissue grafts: Group 1: Lactated Ringer; Group 2: Belzer solution; Group 3: mature coconut water; Group 4: green coconut water; Group 5: modified coconut water. In Group 5, the green coconut water has been modified like the Belzer solution. From each animal we harvested the spleen, ovaries and skin of the back segment. These tissues were preserved for six hours in one of the solutions. Then, the grafts were reimplanted. The recovery of the function of the implanted tissues was assessed 90 days after surgery, by splenic scintigraphy and blood exam. The implanted tissues were collected for histopathological examination. The serum levels did not differ among groups, except for the animals in Group 5, which showed higher levels of IgG than Group 1, and differences in relation to FSH between groups 1 and 2 (p <0.001), 4 and 2 (p = 0.03) and 5 and 2 (p = 0.01). The splenic scintigraphy was not different between groups. The ovarian tissue was better preserved in mature coconut water (p <0.007). the coconut water-based solutions preserves spleen, ovary, and rat skin for six hours, maintaining their normal function.
Highlights
Methods to preserve organs and tissues for transplantation are associated with the suppression of metabolism by hypothermia
There was no difference between the conservation of liquids studied in relation to the evaluation of the function of the splenic implants by scintigraphy, which is a more sensitive method to indicate uptake by spleen cells than the blood tests[14]
The coconut water solution was as effective as the Braun-Collins solution in the preservation of preantral follicles in goats at 4°C and higher temperatures
Summary
Methods to preserve organs and tissues for transplantation are associated with the suppression of metabolism by hypothermia. The blood is replaced by a preservation solution to become tolerant to the organ hypothermia. The composition of preservation solutions is critical to organ tolerance stored in hipotermia[1,2,3,4]. Studies with gametes of various animal species have shown that coconut water (endosperm of Cocosnucifera L.) can be successfully used in the preservation of pre-antral follicles of sheep and goats, and semen of sheep, pigs and humans. This solution was tested as a means for storage and maturation of immature oocytes from bovine ovaries and as culture medium for mouse and bovine embryos 3
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