Preliminary study of artificial synthetic E-selectin inhibits the proliferation mechanism of vascular smooth muscle cells in rats

Abstract

Objective To preliminary study the mechanism of artificial synthetic E-selectin inhibiting the proliferation of vascular smooth muscle cells in rats. Methods Ninety adult male SD rats were randomly divided into 3 groups: a control group, an operation group, and a treatment group (n=30 in each group). The right common carotid artery was made a longitudinal incision and interrupted suture in the operation group and the treatment group, and the control group did not make vascular incision and suture. Artificial synthetic E-selectin and the same amount of saline were injected into the femoral veins of the rats respectively in the treatment group and the operation group after vascular suture. On day 1, 3, 7, and 14 after procedure, ELISA was used to detect the concentrations of serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The vascular tissue at the suture was taken. Western blot and RT-PCR were used to detect the protein level of NF-κB p65 and mRNA expression. Electrophoretic mobility shift assay was used to detect the NF-κB transcriptional activity. Immunohistochemical staining was used to detect the positive cell rate of proliferating cell nuclear antigen (PCNA). Results The concentrations of serum TNF-α and IL-6 and the levels of NF-κB p65 protein and mRNA began to increase at day 1 after vascular suture in the operation group and the treatment group. They reached the peak at day 3, and they were still higher than those in the control group at day 7 (P 0.05). The NF-κB transcription activity in the operation group was higher than that in the treatment group at day 3 after suture (P<0.01). The PCNA positive cells began to express at day 3 after vascular suture in the operation group and the treatment group. The expression of PCNA positive cells in the control group was not obvious or no expression. There were significant differences among the 3 groups (P<0.05). They still expressed high at day 7 and 14 (P<0.01), while the expression of PCNA positive cells was lower than that in the treatment group over the same period (P<0.01). Conclusion Artificial synthetic E-selectin can inhibit the proliferation of vascular smooth muscle cells by inhibiting NF-κB activity. Key words: E-selectin; NF-kappa B; Myocytes, smooth muscle; Cell proliferation; Rats; Vessels