Abstract
ABSTRACT The evaluation of abalone (Haliotis spp.) restocking programs might be improved by the use of microchips attached to seeds [ca. 20–30mmshell length (SL)]. Preliminary studies were conducted to standardize thismethodology and to assess tag retention and the potential impacts of tagging red abalone (Haliotis rufescens) seeds (initial averages: 26 mm SL, 2.5 g total weight) with passive integrated transponder (PIT) tags (9×2mm, Biomark, HPR Plus Reader). Tags were placed in previously made grooves in the shells and glued with a cyanoacrylate adhesive fast cured with sodium bicarbonate. Laboratory trials showed that metabolic rate increased in tagged seeds compared with untagged controls (t = 4.74, P = 0.009) immediately after the tagging procedure, but this effect was not significant after 3 h (t= 1.43, P = 0.23). Food (Macrocystis pyrifera) consumption after 24 h was similar by seeds marked with PIT tags, plastic tags, both tags, and untagged controls (F= 0.12, P = 0.94). Tag retention and survival...
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