Abstract
Abstract Venoms of certain elapid snakes ( Naja , Hemachatus , Ophiophagus , Bungarus , Dendroaspis and Pseudechis ) were dialyzed against distilled water for 48 hr. The dialysates were compared by chromatography and electrophoresis. Unlike viperid and crotalid venoms ( Vipera , Bitis , Crotalus and Bothrops ), elapid venoms are highly dialyzable (30–75 per cent). The toxic dialysates migrated by electrophoresis on Cellogel at pH 7·0 towards the cathode, and could be fractionated by salting-out chromatography. By column chromatography on CM-cellulose, dialysates of Naja naja , Pseudechis collettii and Bungarus fasciatus venom were separated into several fractions having different toxicity. Purified Naja -toxins (Cobrotoxin from Naja naja atra , α-toxin from Naja nigricollis and neurotoxin from Naja naja ) compared by salting-out chromatography showed different behaviour.
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