Preliminary studies of the relation between STAT3 and invasion and metastasis of choriocarcinoma

Abstract

Objective To explore the relationship between the expression of signal transducers and activators of transcription 3 (stat3),the invasiveness and metastasis mechanism of choriocarcinoma cells.Method Choriocarcinoma cell lines(JEG-3 cells and JAR cell)were cultured in vitro,and the human chorionic villi tissues were collected.The invasiveness was assessed by penetration through a matrigel-coated filter in transwell chamber ability between JEG-3 cells and JAR cell.Expression of star3 and p-star3 protein in the human chorionic villi tissues and choriocarcinoma cell lines(JEG-3 cells and JAR ceils)were detected by western blot and immunocytochemistry.Result The expression of stat3 and p-stat3 protein can be found in choriocarcinoma cell lines.The amount of JEG-3 cells penetrating through matrigel-coated filter in transweil chamber was significantly increased,compared with JAR cells(P<0.05).Protein expression level of star3 and p-star3 was significantly increased in JEG-3 cell line,compared with JAR cell line,and it was markedly increased in choriocarcinoma cell lines compared with normal villi tissues(P<0.01).SP immunocytocbemical results also showed that the expression of star3 and p-star3 was significantly increased in JEG-3 cell line,compared with JAR cell line(P<0.05).Conclusions The expression of stat3 and p-star3 protein in choriocarcinonm cells Was higher than that in normal villi tissues,and which in JEG-3 cells Was higher than that in JAR cells. Key words: Transcription factors/ME; Choriocarcioma/ME; Signal transduction; Neoplasm invasiveness; Neoplasm metastasis