Abstract
The nutritional quality of proteins in cereals such as rice,maize, wheat, and other cropsis comparatively low because of a deficiency in some essential amino acids. Lysine is the first restrictive amino acid in these cereals. Lysine-rich proteins were isolated and purified from seeds of the winged bean ( Psophocarpustetragonolobus ), alegume. According to N- and C amino acid sequences of the protein, a pair of primers was synthesized from the deduced nucleotide sequences of both end amino acids. The lysine-rich protein cDNA was cloned by using the primers to run polymerase chain reaction (PCR) according to a cDNA template from a reverse transcription of mRNA of the plant. The purpose of this studywas to transform lysine and thelysine-rich protein cDNA cloned from this legume into a japonica rice variety, ZH8, and study the possibility of improving the nutritional quality of rice by genetic engineering (Liu et al 1993, Sunet al 1998).
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