Abstract
Here we present a protocol for the isolation, purification, and inactivation of the yellow fever virus (YFV) aimed to facilitate high-resolution structure determination using cryo-electron microscopy (cryo-EM). Cryo-EM studies require the concentration of viral particles to be at least 1012 particle/mL. The concentration of the sample was evaluated by a combination of methods, including the plaque-forming assay, spectrophotometry, and protein polyacrylamide gel electrophoresis. High purity of the sample was confirmed by electrophoresis and transmission electron microscopy. The cryo-EM data set was collected and the 2D classification of viral particles was performed.
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