Preliminary screening of intestinal barrier genes associated with porcine epidemic diarrhea virus infection in pigs

Xiaoyi Qi,Haifei Wang,Wenbin Bao,Weiyun Qin,Shenglong Wu,Yafei Xu

doi:10.37496/rbz4920200064

Abstract

To screen intestinal barrier genes associated with porcine epidemic diarrhea virus (PEDV) infection, in the present study we first detected PEDV-infected piglets ( Sus scrofa ) with intestinal damage using quantitative real-time PCR (qPCR) and hematoxylin and eosin (HE) staining. Then, we used qPCR to identify expression differences of intestinal barrier genes between the PEDV-infected and control groups. The results showed that the expression levels of most genes were significantly different between the two groups. Hierarchical clustering and correlation [...]

Highlights

Porcine epidemic diarrhea (PED) is a highly contagious intestinal infectious disease caused by porcine epidemic diarrhea virus (PEDV), with an epidemic peak time in cold climates in the late autumn and early winter, which is mainly characterized by vomiting, diarrhea, and dehydration of piglets (Sus scrofa), resulting in high morbidity and 80-100% mortality (Sun et al, 2012; Li et al, 2012)
We speculated that decreased expression of GLP2 and AQP3 might play an important role in the process of PEDV infection of piglets by reducing the expression of tight junction proteins and disrupting the junctions between intestinal epithelial cells
Porcine epidemic diarrhea (PED) is a highly contagious intestinal infectious disease caused by porcine epidemic diarrhea virus (PEDV), with an epidemic peak time in cold climates in the late autumn and early winter, which is mainly characterized by vomiting, diarrhea, and dehydration of piglets (Sus scrofa), resulting in high morbidity and 80-100% mortality (Sun et al, 2012; Li et al, 2012)

Summary

Introduction

Porcine epidemic diarrhea (PED) is a highly contagious intestinal infectious disease caused by porcine epidemic diarrhea virus (PEDV), with an epidemic peak time in cold climates in the late autumn and early winter, which is mainly characterized by vomiting, diarrhea, and dehydration of piglets (Sus scrofa), resulting in high morbidity and 80-100% mortality (Sun et al, 2012; Li et al, 2012). Individual death and decreased performance caused by this virus cause huge economic losses to the pig industry, while the use of drugs and vaccines and related biosafety problems incur further economic losses (Song and Park, 2012). The main means of prevention and control of PED is vaccination; the genetic variation of PEDV strains causes enhanced virulence of variant strains or make effective immunization using vaccines difficult (Li et al, 2012); it is, urgent to find a permanent cure. Genetically enhancing piglet resistance to PEDV might be the most effective and fundamental way to control PEDV infection in the long run

Methods

Results

Conclusion