Abstract
Lactic acid bacteria (LAB) alone or with special adjunct probiotic strains are inevitable for the preparation of specific functional foods. Moreover, because of their growth and metabolism, final products are preserved for a certain time. Thus, in this work, growth and metabolic activity of novel animal origin isolates and culture collection strains of Bifidobacterium spp. were investigated. The influence of milk media (reconstituted or ultra-high-temperature (UHT) milk), compared with synthetic modified Wilkins–Chalgren (WCH) broth under aerobic conditions was investigated. All tested bifidobacterial strains (n = 10) were grown well (1–2 log colony-forming units (CFU)/mL for 24 h at 37 °C) in all substrates and levels higher than 5 log CFU/mL remained during the cold storage period. Generally, different substrates determined almost the same maximal population densities (MPD) after 24 h that range within the average values of 8.96 ± 0.43 log CFU/mL, 8.87 ± 0.52 log CFU/mL, and 8.75 ± 0.54 log CFU/mL in reconstituted milk, UHT milk, and WCH broth, respectively. After 28 days of storage, the pH levels in milk media and broth were reduced to 4.50–5.60 and 4.60–4.90, respectively, representing a decrease of 0.8–2.13 units.
Highlights
Genus Bifidobacterium is one of the most beneficial probiotic microorganisms and one of the most predominant cultures in the human colon and breast-fed infants [1]
Bifidobacterium is a genus of Gram-positive, catalase-negative, non-motile, and non-spore-forming bacteria
The reported optimal temperature for the growth of human bifidobacterial strains is between 36 ◦ C and 38 ◦ C [2]; for species of animal origin, it is slightly higher (41–43 ◦ C) and may be even as high as 49.5 ◦ C, as shown for B. thermacidophilum [4]
Summary
Genus Bifidobacterium is one of the most beneficial probiotic microorganisms and one of the most predominant cultures in the human colon and breast-fed infants [1]. Bifidobacterium is a genus of Gram-positive, catalase-negative, non-motile, and non-spore-forming bacteria. They are obligate, partly facultative anaerobes belonging to the Actinobacteria phylum, and are naturally predominant components of the intestinal microflora, presenting up to 20% of the fecal bacteria in adults and 80% in infants [3]. The reported optimal temperature for the growth of human bifidobacterial strains is between 36 ◦ C and 38 ◦ C [2]; for species of animal origin, it is slightly higher (41–43 ◦ C) and may be even as high as 49.5 ◦ C, as shown for B. thermacidophilum [4].
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