Preliminary Screening and Analysis of Biomembrane Permeable Compounds in Herbal Medicines: Hollow Fiber Liposome Microscreening Combined with HPLC

Abstract

An advantageous method based on hollow fiber liposome microscreening (HFLMS) combined with high-performance liquid chromatography (HPLC) has been developed and used in preliminary screening and analysis of biomembrane permeable compounds from herbal medicines (HMs). In the method, a liposome hollow fiber was prepared as a screening tool; permeable compounds that could specifically bind to liposomes were screened from herbal extract to liposome in the pores and lumen of hollow fiber, then permeable compounds were dissociated from liposomes and analyzed by HPLC. The chromatographic separation was applied with a Zorbax Eclipse XDB-C18 column by a gradient elution using acetonitrile, methanol, and phosphoric acid aqueous solution as the mobile phases. In the study, influencing factors of the screened target compounds were investigated and optimized. Under optimum conditions, the surface properties of liposome hollow fibers were characterized, the nonspecific binding between the active center of hollow fiber and the bioactive components were investigated, the repeatability of this method was tested. And eight permeable compounds of flavonoids and anthraquinones from HMs were preliminarily screened and analyzed by HFLMS-HPLC. The results demonstrated that new method is a simple, fast, effective, and reliable method for preliminary screening and analyzing biomembrane permeable ingredients in HMs, and it can be used in screening other permeable compounds in HMs.