Preliminary Research of Main Components of Dll4/ Notch -VEGF Signaling Pathway Under High-Glucose Stimulation In Vitro

Abstract

Objective: To set up high-glucose stressed cell model and investigate the expression of main components of Dll4/Notch-VEGF signaling pathway under high-glucose stimulation. Method: The cells (human umbilical vein endothelial cells, HUVECs) model under high-glucose condition were set up at first, and then the expression of Dll4, Notch1, Notch4 and VEGF in HG-stressed cells with or without Notch pathway blockage were analyzed through RT-PCR and western blot. To observe the cell migration, we also evaluated the Transwell assay. Results: HUVECs stimulated with 30mmol/L HG was chosen as cell model. Results from RT-PCR and western blot showed that HG stimulation induced Dll4, Notch1 and VEGF expression and down-regulated Notch4. The expressions were reversed after Notch pathway blockage, meanwhile, the blockage of Notch pathway inhibited cell migration under HG condition. Conclusions: Function of Notch4 is worth further researching under HG stimulation. Combination therapy by blocking Dll4/Notch and VEGF pathways might provide us a new way for anti-neovascularization. Funding Statement: This work was supported by the research program of Health and Family Planning Commission of 212 Sichuan Province, China (17PJ534). Declaration of Interests: The authors have no conflicts of interest to declare. Ethics Approval Statement: This study performed in accordance with the Declaration of Helsinki and have been approved by ethics committee of Teaching Hospital of Chengdu University of Traditional Chinese Medicine.