Preliminary Phytochemical Analysis and Isolation and Identification of active principle components of petroleum ether extract from leaves of Lawsoniainermis L.

Abstract

Background: Lawsoniainermis L belongs to the Lythraceae family, generally known as Henna, cultivated for its leaves although stem, bark, roots, flowers and seeds. They were used in traditional medicine to treat a variety of ailments as rheumatoid arthritis, headache, ulcers, diarrhea, leprosy, fever, leucorrhoea, diabetes, cardiac disease. They were used for dye making. Aim: The present study focus on preliminary phytochemical analysis and determination of bioactive components from the leaf of Lawsoniainermis L. Methods: Preliminary phytochemical analysis and identification of active components by GC/MS were done. Result: The preliminary phytochemical analysis reveals that the aqueous and petroleum ether extracts from leaf of Lawsonia inermis Lconsists of alkaloids, anthraquinones, flavonoids, phenols, reducing sugars, saponins, steroids, tannins, and terpenoids.GC/MS analysis reveals that petroleum ether extracts consists of phthalic anhydride, 4-methylformanilide, acetamide, N-(2-methylphenyl)-, 3-hydroxycarbofuran, 2-hydroxy-1,4-naphthalenedione, dodecyl acrylate,3,7,11,15-tetramethyl-2-hexadecen-1-ol, trans-geranylgeraniol, n-hexadecanoic acids, 9,12,15-octadecatrienoic acids, (Z,Z,Z)-octadecanoic acids, phytol, acetate, cis-13-eicosenoic acid, cyclohexadecane-1,2-diethyl-, 4,8,12,16-tetramethylheptadecan-4-olide, eicosane, tetratriacontane, supraene, 1,6,10,14-hexadecatetraen-3-ol, 3,7,11,15-tetramethyl-, (E,E)-, stigmastan-3,5-diene, triacontane, and vitamin E. Conclusion: This study shows that Lawsoniainermis L. leaves contain bioactive phytochemicals that may serve as a biocompatible and eco-safe agent in formulation of drugs, natural dyes in colouration of textile materials and other purpose.